Or scan up to 384 wells at any time to determine the best assay conditions. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). 190. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. 1. Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. (Molecular Devices cat. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. x. Site. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. as well as all other Molecular Devices' microplate readers. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 306 plate air cal. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Unparalleled performance on a personalized platform. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. The luminescence was measured in a SpectraMax. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Features. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. All trademarks used herein are the property of. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Primary considerations when choosing a microplate reader are throughput and flexibility. MCF7 cells treated with. The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. TNF standard curve. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. 500 OD ± 3. 96-well microplate compatible Well read area range: D4. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 0. Users can choose to process all plates within the magazine or select a specific number. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. x. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). The color touch screen provides a visual user interface, making programming fast and intuitive. S. The CellTiter-Glo assay was used to measure ATP. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. 000 OD ± 1. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. $542. Absorbance Linearity: 2. 000 – 4. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. Published Dec 16,. x. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. Top reading gives greater sensitivity for 96 well plates, unless you have cells adhering to the bottom of the well. #CA961) Methods. 17,000 cells. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. 2. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Page 1 of 8 . The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Lucid Scientific. Contents of wells were pipetted up and down several times to mix. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. The CellTiter-Glo assay was used to measure ATP. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. adjust the reader. Bay Gray powder coated metal back plate, column, and base. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. one 96-well sample plate can be controlled from 15–40°C,. Mode Plate Reader. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Figure 1. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. #C1500) Control standard endotoxin (ACC cat. 0200-6262. External Dimensions without lid (mm). style. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. . Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Loading Buffer was also added to a set of cell-free wells for background controls. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Click Add to create the plate. Can read 96 well plates (optional 384 well). Molecular Spectroscopy Consumables & Accessories Thermal Analysis. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Sample by eye within . D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Reader Documentation Version No. 7°C and the bottom to 36. It also integrates with the powerful SoftMax Pro Software for. 1 mL/well of media. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. DynaPro ™ Plate Reader III. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Optional 16-way manifold. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Molecular Devices (UK) Ltd. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. We would like to show you a description here but the site won’t allow us. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Mix samples in microplate wells by user-programmable Automix feature. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. Cross-talk <0. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. BMG Labtech - THERMOstar. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. to each well. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. UV absorbance detection. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. (one set per assay) to clear 96-well microplates. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Figure 2-23: Bottle holder To install the bottle holder: 1. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 1 – 2. Figure 3. Fluorescence Microplate Reader Comparison Chart. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Browse for different fluorescent readers below and compare across different instrument suppliers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. When a. Enter the name of the plate type i. Growth medium was then replaced with fatty acid uptake solution. B. Make sure toPlate reader instructions Computer. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Component A (2 vials*)Figure 1. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 1 fmol luciferase per well. 1 x plates; Instrument dimensions. measurements in compatible Molecular Devices® microplate readers. x. 130009WMicroplate Readers. 000 to 2. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Integrated stacker option. Microplate Readers include the M3, M4, M5, and M5e models. 00. Range of 96, 384-well SBS microplates . Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. x. High-throughput, automated dynamic light scattering. 000 to 2. Molecular Devices. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Compare Tool. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Packed 25 plates per sleeve, 2 sleeves per case. It features a modular design that allows users to add or change detection cartridges according to their needs. Molecular Devices has provided scientists with . Inconsistent results: cannot determine whole well confluence – well after well. Janet got excellent results using them and they are only ~$1. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 96-, 384-, and 1536-well microplates. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. UV absorbance detection. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Some will only support one well density (e. Temperature control range from Ambient +4º to 50ºC. Optimized for fluorescence microplate readers. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. 046 to 0. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. 4 decades . This reader's dual monochromators for. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 96-well plates - Filter plates with 0. minutes and begin reading microplates with seven mouse clicks. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Microplate Spectrophotometers. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Other. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. In SoftMax Pro, Dual Read mode was selected. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 01. Dynamic range > 6 decades . Self-righting base, up to 40 degrees past center without dispenser. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. No accessories are needed and SoftMax® Pro Software. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Learn more. and glow luminescence modes in 96- to 384-well plates. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. x. Temperature control: 4°C above ambient to 45°C. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. • Remove media from each well using a blue pipette tip being careful as not to remove cells. Microplate Readers include the M3, M4, M5, and M5e models. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. 100 fmol 1536-well (8 µL) 0. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 312 gain calibration failed. Use standard plate unless you see your type listed. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 2. 25% and 0. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Packed 25 plates per…. Our. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Thermomax model provides thermal control and vertical shake, perfect for. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. Linear, Orbital Slow, and Orbital Fast. (96- and 384-well with pipetting) 6-384 (96- and 384-well). Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The soil was amended with either 1. Your applications, your modes, your choice. x. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. . 5 OD at 492 nm Computer interface USB 2. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. UV Plate 96 Well, Flat Bottom, Cs 50. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. FlexIII. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. SpectraMax ID5 Plate Reader (Molecular Devices). #E010) Pyroplate 96-well microplate (ACC cat. More Product Information. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Shaking: Linear, orbital, and double orbital. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. 0% ± 0. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Published Dec 16, 2020 | Updated Nov 07, 2022. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. The StakMax stacker also allows users to feed plates into the loading area manually. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. The plate reader can measure multiple readouts, including UV and. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Validation made easy -1) Select the correct plate and the wells that you want to measure. 1. Polypropylene deep well plates, round bottom. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. µl) was added to each well and incubated at 37°C for 1 h. C. 5 fg per well to 0. #CA961) Methods. Step 3: Click “Settings” and a screen will appear (Figure 1). Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. Supplier: Molecular Devices. 9. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Figure 3. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. Order Now. Gemini EM Reader scans. Please refer to the Obsolete Microplate Reader Models for more information. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. For biochemical assays, the washer removes unbound material and unreacted reagents. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. 314 low lamp level warning. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Therefore you need to ask a physicist. Uncontrolled Copy for Training and Reference Purposes Only . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 9. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. The IC50s by cell rounding and LysoTracker. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Plate. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. 2. The variations include adjustable. with Quartz ABS Plastic. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. (96- or 384-well) simultaneously and with high temporal resolution. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. x. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Usage/Application: Lab. 3. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. *For Corning UV plates, mean values at 260 nm ranged from 0. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. 9000-0756. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Z. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The plate reader optics are tunable for. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Temperature variation ± 0. No tools required to assemble. Plate Shaking. . For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. We would like to show you a description here but the site won’t allow us. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. It also integrates with the. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Corning. % transmittance of plates used in a plate reader. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Step 2: Add sample to well – antigen within the sample binds to the capture antibody.